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Absorbance Apparatus

 

  • Materials:
 
 
 
 
 
 
 
 
 
 
 
 
  • Microtiter Plates:
 

Nunc-Immuno Module F8 96-well microtiter plates (4-69078, Thermo Scientific™) were employed for absorbance measurement due to their excellent optical quality. Clean microtiter plates were used in order to reduce optical errors. F8 plates (F denotes flat-bottom) were chosen as they allow the light beam emitted by the spectrophotometer to travel a straight path.

 

  • Microplate Reader:
 

Modulus™ II Microplate Reader made by Turner BioSystems, Inc. is a high-throughput spectrophotometer designed for microtiter plates. A microtiter plate shaker is integrated within the microplate reader.

 

Absorbance of the chromophore was measured at wavelength 450 nm (mainly Tartrazine) and 600 nm (mainly Trypan Blue). The light beam direction for absorbance measurement was from top to bottom. Mixing was set at 300 rpm in the orbital mode for 10 minutes.

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